Storage and Handling

Store lyophilized 5-Amino-1MQ at -20°C, protected from light and moisture. In freeze-dried form, the compound stays stable for extended periods under proper storage. After reconstitution, keep refrigerated and use within a short window under sterile conditions. Handle only in a controlled laboratory setting.

5-Amino-1MQ Research

5-Amino-1-methylquinolinium (5-Amino-1MQ) is a methylquinolinium derivative built around a quinolinium core with an amino group at the 5-position and a methyl group at the 1-position. It is studied as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme linked to metabolic and oncogenic pathways in research models.

The sections below summarize what published laboratory studies report about this compound and its molecular target.

Mechanism of Action

5-Amino-1MQ inhibits NNMT, the enzyme that transfers a methyl group from SAM onto nicotinamide. NNMT sits between NAD+ metabolism and one-carbon methylation metabolism, pathways that support redox reactions, DNA repair, and transcriptional regulation. [1]

In cultured mouse adipocytes, exposure to a methylquinolinium NNMT inhibitor raised intracellular NAD+ and SAM and lowered lipogenesis. [1] Selectivity testing indicated the scaffold did not inhibit related SAM-dependent methyltransferases or NAD+ salvage enzymes. [1]

NNMT as a Metabolic Research Target

Independent research groups have studied NNMT inhibition in diet-induced obese (DIO) mouse models. In one program, a separate NNMT inhibitor reduced 1-methylnicotinamide levels and improved insulin sensitivity and glucose tolerance in DIO mice, with these effects absent in NNMT-knockout animals. [2]

In DIO mice, 5-Amino-1MQ was reported to reduce body weight and white adipose mass and to lower plasma cholesterol, without a measured change in food intake. [1] The authors framed the observed metabolic shifts as a direct effect on lipid handling in the model rather than appetite suppression. [1]

Adiposity and Microbiome Studies

Paired with a low-fat diet, 5-amino-1-methylquinolinium was used to study whole-body adiposity and weight change in DIO mice, moving several measures toward age-matched lean controls within the study window. [3] The same work profiled shifts in the cecal microbiome associated with treatment. [3]

Oncology Research Applications

Beyond metabolism, 5-Amino-1MQ has been examined in cell-based oncology research. In a cervical cancer cell line (HeLa), the compound reduced cell proliferation in a concentration- and time-dependent way and lowered expression of phospho-Akt and SIRT1, two proteins tied to tumor progression, while a non-cancer reference cell line was less affected. [4]

Pharmacokinetics and Distribution

Pharmacokinetic work in mice reported systemic exposure and distribution of 5-Amino-1MQ to metabolically active tissues, including adipose, muscle, and liver, after subcutaneous compound application. [5] In the same 28-day study, DIO mice showed reduced body weight and fat mass gains alongside improved glucose tolerance and attenuated hepatic steatosis. [5] Work on tissue-specific targeting and metabolic stability continues in research settings.

Certificate of Analysis (COA) for Every Batch

A Certificate of Analysis (COA) is a document that verifies a compound’s identity, purity, and batch quality through independent laboratory testing. Every compound from BioLongevity Labs ships with a COA tied to its specific batch, so researchers can confirm exactly what they received before it enters a protocol.

Each COA reports results from third-party laboratory analysis, including:

• High-performance liquid chromatography (HPLC) for purity, typically confirmed at 99% or higher
• Liquid chromatography mass spectrometry (LC-MS) for molecular identity and mass confirmation
• Sterility and endotoxin screening where applicable
• Chemical contaminant and residual solvent checks

COAs are sourced from independent certified labs rather than in-house testing alone, giving researchers a verifiable record of molecular integrity for each batch. All compounds are supplied for research use only.